The work of the group of Prof. Siegfried Knasmüller covers the area of genetic toxicology. The topics are widespread and comprise the contamination of environmental compartments (air, water, soils), with genotoxic carcinogens, the detection of dietary factors which protect against cancer and DNA damage, as well as studies concerning occupational exposures which cause DNA instability and cancer.
Recent activities concern the effects of obesity on DNA stability and the investigation of adverse (DNA-damaging) properties of widely used synthetic drugs (cannabinoids, cathinones). A new project concerns the search for metabolically competent cell lines for mutagenicity testing.
Methods established in the Knasmüller group:
a) Bacterial mutagenicity tests;
b) Cytogenetic tests with plants;
c) Comet assays in vitro and with rodents, and humans;
d) Human biomonitoring protocols such as the micronucleus (MN) assay with lymphocytes and exfoliated cells and chromosomal aberration assays;
e) Investigations concerning preneoplastic lesions in different organs e.g. aberrant crypt foci (ACF) in the colon
Recommendations and quality criteria for micronucleus studies with humans
Nersesyan A, Kundi M, Fenech M, Stopper H, da Silva J, Bolognesi C, Mišík M, Knasmueller S.
Mutat Res Rev Mutat Res. 2022 Jan-Jun;789:108410. doi: 10.1016/j.mrrev.2021.108410.
Induction of DNA damage as a consequence of occupational exposure to crystalline silica: A review and meta-analysis
Wultsch G, Setayesh T, Kundi M, Kment M, Nersesyan A, Fenech M, Knasmüller S.
Mutat Res Rev Mutat Res. 2021 Jan-Jun;787:108349. doi: 10.1016/j.mrrev.2020.108349
Genotoxic activities of wastewater after ozonation and activated carbon filtration: Different effects in liver-derived cells and bacterial indicators
Misik M, Ferk F, Schaar H, Yamada M, Jaeger W, Knasmüller S, Kreuzinger N.
Water research 2020 Aug 21;186:116328. doi: 10.1016/j.watres.2020.116328
Environmental risk assessment of widely used anticancer drugs (5-fluorouracil, cisplatin, etoposide, imatinib mesylate)
Misik M, Filipic M, Nersesyan A, Kundi M, Isidori M, Knasmüller S.
Water research 2019 Nov 1;164:114953. doi: 10.1016/j.watres.2019.114953.